President Donald Trump directly asked about a Ukrainian investigation into his Democratic rival Joe Biden, a top US diplomat has unveiled.
Bill Taylor, the acting ambassador to Ukraine, told an impeachment inquiry that a member of his staff was told Mr Trump was keen to push for the probe.
Mr Trump says he does not recall making the remark. He denies any wrongdoing.
Mr Biden hopes to run against Mr Trump next year. It is illegal to ask foreign entities for help to win an election.
He is accused of withholding US military aid to Ukraine in order to pressure the country’s new president to publicly announce a corruption inquiry into Mr Biden.
Mr Trump has called the inquiry a “witch-hunt”.
During a detailed opening statement, Mr Taylor said a member of his staff had overheard a telephone call in which the president inquired about “the investigations” into Mr Biden.
The call was with Gordon Sondland, the US ambassador to the European Union, who reportedly told the president over the phone from a restaurant in Kyiv that “the Ukrainians were ready to move forward”.
After the call, the staff member “asked ambassador Sondland what President Trump thought about Ukraine”, Mr Taylor said.
Mr Taylor said: “Ambassador Sondland responded that President Trump cares more about the investigations of Biden.”
Meanwhile observers and former officials have drawn attention to the security implications of making the call from a restaurant, potentially exposing the conversation to eavesdropping by Russian intelligence.
When asked about Mr Sondland earlier this month, the president had said: “I hardly know the gentleman.”
Responding to queries from reporters after the hearing, Mr Trump said: “I know nothing about that, first time I’ve heard it.”
He said he recalled Mr Sondland’s testimony, in which the diplomat said he spoke to the president “for a brief moment” and Mr Trump had “said no quid pro quo under any circumstances”.
He did not recall the phone call Mr Taylor described, “not even a little bit”, and “in any event it’s more second hand information”, he said.
The impeachment inquiry has been going on for more than a month – but all previous hearings were private, with reports based on leaks and sources speaking to the media.
Wednesday’s public hearings were the first time the public heard from witnesses directly and a chance for Democrats and Republicans to win over voters.